The need for high-throughput sample preparation in proteomics

In proteomics applications, effective analyte capture is essential to ensure reliable downstream results. However, this can be challenging due to the complexity of sample preparation, a key step for achieving desired results. Solid phase extraction (SPE) provides a reliable method for sample clean up, but can be a daunting task. Manual steps in SPE workflows introduce potential variability, which can compromise consistency.

Challenges with traditional processing techniques

Standard processing methods, like vacuum pressure and centrifugation, often lead to issues, including filter failures. These failures can arise due to:

• Limited, uneven vacuum distribution across samples
• Transverse forces during centrifugation

Both factors contribute to sample losses, affecting workflow reliability.

Resolvex^® instruments: A positive pressure solution

Resolvex instruments address these issues by applying an even air flow across channels. The unique air manifold design ensures parallel pressure is applied to each individual column or well whether using a full or partially filled well plate or column rack.

Automation and reproducibility with the Resolvex A200

Designed for automation and reproducibility, the Resolvex A200 allows users to define air pressure profiles with precise pressure gradients and control solvent dispensing steps via the touch screen at will. When a protocol is determined satisfactory, the defined method may be saved for future use. With the touch of a button, users can confidently process up to 96 samples simultaneously. Automatable sample preparation significantly reduces variability associated with manual tasks like pressure control and pipetting.

1. An automated liquid dispenser accurately delivers solvents using an 8-channel dispense head. Up to 11 solvents can be connected to the A200 at once allowing multiple sample preparation protocols to be setup without hassle.

2. Intuitive touchscreen for programming dispensing steps and pressure profiles. Multiple sample preparation methods may be developed and saved on the instrument for easy access.

3. Positive pressure manifold enables maximum reproducibility with precise flow controls for parallel processing of wells or columns.

4. High capacity waste reservoir for a direct drain to waste bottle simplifies disposal processes.

Narrow Bore Extraction^™ (NBE^™) format

Narrow Bore Extraction (NBE) formats are produced with microparticulate sorbents to ensure effective analyte cleanup. The unique design of NBE includes a separate reaction vessel to enable on-column reactions such as digestion or hydrolysis, with or without incubation. The patented air-lock design prevents premature sample breakthrough until positive pressure is applied. The narrow bore outlet allows low volume elution for higher analyte concentration.

AffinEx^™

Monoclonal antibody (mAb) research is one of the major forces behind discovering novel and targeted therapeutics for unmet medical needs. Protein purification via affinity capture has proven useful in various scales of sample processing, but due to the varying sample types, volumes and contents, purification can be cumbersome to navigate at benchtop scales.

AffinEx Protein A provides a simple solution for antibody purification with stability under ambient conditions, Processing can be done for up to 96 samples for NBE and 1ml columns using the Resolvex A200. Leveraging the Resolvex A200, antibody purification protocols become reproducible for various columns formats.

Automating sample preparation

Automated SPE sample preparation using positive pressure processing may expedite sample cleanup providing high-throughput solutions for labs. The ability to automate otherwise tedious manual steps has given labs confidence in their sample preparation with reduced variability. Scientists rest easy with automation spending their time on other challenging tasks by automating their SPE sample preparation.

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