Thermo Fisher Scientific Announces New Chemiluminescent Option for Western Blot Detection

Thermo Fisher Scientific, the world leader in serving science, today introduced Thermo Scientific Pierce ECL Plus Substrate for Western blotting detection. The Pierce® ECL Plus Substrate is a highly sensitive, acridan-based reagent used for the chemiluminescent or chemifluorescent horseradish peroxidase detection of immunoblots.

The Pierce ECL Plus Substrate detects low picogram level of antigen, which can be visualized on X-ray film or with an imaging system. The long-lasting signal generated using the Pierce ECL Plus Substrate can be detected easily for up to five hours, and up to 20 hours with longer exposure times. The substrate is compatible with both PVDF and nitrocellulose membranes.

The substrate is available in two sizes, with reagent sufficient for 250 or 1,000 squared centimeters of membrane. For more information on the Pierce ECL Plus Western Blotting Substrate, please visit the company article page.