Thermo Fisher Scientific introduces fast, automated Plasmid DNA Purification Kit

Thermo Fisher Scientific has introduced the Applied Biosystems^™ MagMAX^™ Pro HT NoSpin Plasmid MiniPrep Kit to address the critical need for rapid and automated plasmid DNA isolation, enabling scientists to conduct small-scale experiments quickly and affordably.

Purifying plasmid DNA, essential for researching therapeutics for diseases such as infectious diseases, cancers, and monogenic disorders, can now be done more efficiently.

Exceptional for workflows requiring high-quality plasmid DNA, the MagMAX Pro HT NoSpin Kit is one of the fastest plasmid purification kits available. From just 1 milliliter of fresh sample culture, this innovative approach allows for 96 minipreps to be completed in under 35 minutes, including initial set-up, on a Thermo Scientific^™ KingFisher^™ instrument. The kit also integrates effortlessly on high-throughput systems ensuring scalability from benchtop to large scale operations.

As the demand for plasmid-based research grows, the transition from research to applied studies is becoming more seamless, no longer hindered by bottlenecks in plasmid DNA isolation. The new MagMAX Pro HT NoSpin Kit completely eliminates manual and time-consuming steps such as initial cell pelleting and offline lysis requiring repetitive centrifugation or filtration.

What’s more, the MagMAX Pro HT NoSpin Kit uses novel magnetic bead technology and ecofriendly purification chemistry, minimising reagent waste and costs, making advanced research accessible to more laboratories.

“Laboratories of all sizes are seeking solutions that expedite the pace of research and remove bottlenecks and inefficiencies that impede the development of future therapeutics,” said Kevin Lowitz, vice president, sample preparation, Thermo Fisher Scientific. “By automating the plasmid purification process and requiring only a small amount of overnight culture, we enable scientists to conduct numerous experiments efficiently. This allows them to save valuable time and resources, seamlessly scale up their projects, and advance research more rapidly."

For labs requiring larger volumes of plasmid DNA, Thermo Fisher offers the Thermo Scientific^™ KingFisher^™ PlasmidPro Maxi Processor. PlasmidPro automates the purification process from culture to plasmid DNA, requiring no centrifugation steps or manual intervention. This can streamline lab workflows and reduce researchers’ hands-on time. It can produce transfection-grade, high-purity plasmid DNA, with over 80% in the supercoiled form, from 150 milliliters of liquid bacterial culture in only 75 minutes, improving lab efficiency. PlasmidPro is now commercially available globally.

Key applications

The MagMAX Pro HT NoSpin Kit is designed for biotech and pharma companies, contract research organisations, contract development and manufacturing organisations as well as academic research institutions, that require high-quality, transfection grade, plasmid DNA for the early screening phases of therapeutic and vaccine research.

Key benefits

• Increased efficiency: the MagMAX Pro HT NoSpin Kit produces quality plasmid DNA demonstrating greater transfection efficiency than manual methods. This ultra-high efficiency allows for a wide range of downstream applications, including transfection into sensitive cell lines, preparation of short hairpin vectors, enzymatic modifications, cloning, restriction digestion, sequencing and in vitro transcription and translation. 
• Seamless scalability: designed to be combined with the KingFisher Apex^™ or Flex^™ instruments, the MagMAX Pro HT NoSpin Kit facilitates easy scaling from small experiments to larger projects, ensuring consistent results across different research phases. 
• Fast purification: produces high-quality purified plasmid DNA in less than 35 minutes, faster than most plasmid purification systems available. 
• Easy-to-use design: requires minimal hands-on time for researchers due to the dual magnetic beads and does not require centrifugation or vacuum filtration, reducing the need for manual intervention. 
• High-volume, high-purity outputs: yields up to 20 micrograms of high-copy plasmid DNA per 1 milliliter of bacterial culture and delivers transfection-grade purification with low endotoxin levels. 

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