Thermo Fisher Scientific Introduces Kit for Subcellular Protein Fractionation

Thermo Fisher Scientific, the world leader in serving science, introduces a kit for easy and rapid subcellular protein fractionation. The Thermo Scientific Subcellular Protein Fractionation Kit enables the stepwise separation and preparation of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein extracts from mammalian cultured cells or tissue for protein localization studies or enrichment of specific cellular compartments.

The simple protocol is completed in less than three hours and requires no ultracentrifugation over gradients during any of the steps. The subcellular extracts obtained are compatible with a variety of downstream applications including protein assays, Western blotting, electrophoretic mobility assays and enzyme activity assays.

The Subcellular Protein Fractionation Kit includes four extraction buffers, a stabilized nuclease and the popular Thermo Scientific Halt Protease Inhibitor Cocktail. Each kit has sufficient reagents to fractionate 50 cell pellets of 20 µl each, equivalent to approximately 2 grams of cell paste or tissue. The kit can be stored at -20°C for up to one year. For more information on the Subcellular Protein Fractionation Kit, visit www.thermo.com/pierce or call +1-815-968-0747.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.