Thermo Fisher Scientific Introduces New Tools for Studying GTPases and Large G-protein Subunits
10 Feb 2011Thermo Fisher Scientific introduces the Thermo Scientific Pierce GTPase Enrichment Kit for profiling small GTPases and large G-protein subunits. The kit provides the reagents for the broad enrichment of GTP-binding proteins from tissues, cells and subcellular proteomes.
The Pierce® GTPase Enrichment Kit expands on the use of novel desthiobiotin-nucleotide probes that are also found in the new Thermo Scientific Pierce Kinase Enrichment Kits. The nucleotide is attached to desthiobiotin, a biotin analog developed by ActivX Biosciences. The nucleotide binds to the active site of the GTP-binding protein, and the desthiobiotin tag allows for capture and enrichment of the bound protein. The desthiobiotin tag binds less tightly to biotin-binding proteins, making the capture bond easily reversible by biotin displacement, low pH or heat. The isolated proteins then can be further studied by Western blot analysis or mass spectrometry.