Top ten tips for producing 13C, 15N protein in abundance

What could be easier than overexpressing an E. coli protein in E. coli? You don’t have to be an old hand at protein expression to know that this can often be more difficult than it sounds. Cambridge Isotope Laboratories (CIL) tested its skills recently with DsbA, a 20 kDa protein that catalyzes disulfide bond formation in the E. coli periplasm. In this application note, find top ten tips for expressing recalcitrant proteins.