Ultrafast, High-Resolution Fluorescence Image Recording - New High-speed Camera System for Live Cell Research

Speed is often the deciding factor for successful imaging in demanding high resolution image documentation of living cells, molecular processes and rapidly fading fluorescence specimens. The monochrome digital camera Leica DFC360 FX is designed to produce brilliant images at maximum temporal resolution. Thanks to state-of-the-art CCD technology, the new camera system achieves maximum frame rates of 20 fps for full frame up to more than 100 fps in binning mode. The highly sensitive sensor and active Peltier cooling ensure a high dynamic range even for low light intensities. The Leica DFC360 FX is the ideal solution for excellent results in live cell imaging.

The Leica DFC360 FX will convince even the most demanding user with its performance on PC and MAC systems, intuitive Leica Imaging Software LAS and LAS AF and optimal integration into high-end fluorescence systems of Leica Microsystems such as Leica AM TIRF MC or Leica AF6000 LX. Experiments such as z-stack, multi-channel fluorescence with overlay, 3D deconvolution, time controlled analysis and many other applications are controlled swiftly and effectively. Ultrafast and uncomplicated data transmission is ensured by the Firewire 1394b interface.

The light sensitivity and the frame rate can be further enhanced by binning (over 100 fps with 8x8 binning, for example). Binning and ROI can be used, as can multi-channel experiments with different amplifications, in the fast overlapping mode. The active Peltier cooling minimizes the dark current and guarantees noise-free images even at low light intensities. With shutter speeds of 4μs to 10 minutes and up to tenfold signal amplification, the Leica DFC360 FX offers maximum flexibility. The Leica DFC360 FX proves its full performance potential with ultrafast fluorescence filter wheels, high-precision light sources and perfect sequencer synchronization, satisfying even such demanding applications as Ca++ and FRET experiments.