Visualizing DNA for long-read sequencing by moles, not mass

Traditional measurements during NGS library preparation include the determination of the sample mass within specific size ranges. This analysis is easily performed with Agilent automated electrophoresis instruments, which provide visual results in the form of a digital gel image and electropherogram. While this representation has been widely used for quality control of sheared gDNA and the final NGS library, examining the molarity of a sample may provide a better visual representation of the number of sequencing reads that can be produced by a sample, especially for long-read sequencing. Explore Agilent's analysis of high molecular weight samples using the Agilent Femto Pulse system and the accompanying ProSize data analysis software.