3M™ Harvest RC Centrate Chromatographic Clarifier
3M™ Harvest RC Centrate Chromatographic Clarifier is a single stage single-use chromatographic clarification solution designed to effectively clarify modern cell culture centrates. Centrate clarification using 3M™ Harvest RC Centrate Chromatographic Clarifier may provide many benefits including process compression, high product recovery, and improved process economics.
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3M™ Harvest RC Centrate Chromatographic Clarifier encapsulates innovative synthetic fibrous anion exchange (AEX) Q-functional chromatography media combined with an asymmetric Q-functional polyamide membrane with a 0.2 μm qualifying zone. This enables effective single-stage clarification of CHO cell culture centrates produced by conventional and single-use conical plate (e.g., disc stack) centrifuges. Soluble and insoluble impurities are captured by electrostatic interactions with a positively charged ligand immobilized on fiber matrix, as well as a downstream polyamide membrane. This results in efficient retention of a wide range of particles that have not been effectively separated by centrifugation or are produced by cell shear during centrifugation. Specifically, the anion exchange mechanism enables efficient capture of impurities <0.5 μm that are difficult to capture with traditional depth filtration and membrane filtration.
Key features and benefits:
- Synthetic, functionalized fibrous media provides effective soluble impurity removal in centrate, including DNA and cell shear related sub-micron particle reduction
- Single-stage centrate clarification for process compression and streamlining of process development
- Predictable performance scalability for consistent clarification at various process scales
- High product recovery (>95%) and high-capacity capsules for improved process economics
- Alkaline resistant capsules for peruse sanitization
- Provides comparable effluent quality to 3M™ Harvest RC Chromatographic Clarifier for a modern clarification platform