3M™ Harvest RC Chromatographic Clarifier
The 3M™ Harvest RC Chromatographic Clarifier is a single stage single-use chromatographic clarification solution designed as an efficient option for harvesting and clarification of modern cell cultures. Clarification using 3M™ Harvest RC Chromatographic Clarifier may provide many benefits including fewer processing steps, higher yield and lower production time and costs.
3M™ Harvest RC Chromatographic Clarifier family of products
3M™ Harvest RC Chromatographic Clarifier process train
3M™ Harvest RC Chromatographic Clarifier platform infographic
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3M™ Harvest RC Chromatographic Clarifier encapsulates innovative synthetic fibrous anion exchange (AEX) chromatography media and a 0.2 μm polyether sulfone (PES) membrane. This enables a single-stage clarification process of low to high-density cell culture (>40 million cells per mL) with high recovery and high separation fidelity of soluble and insoluble impurities.
Cells are bound inside the media by electrostatic charge interaction with the AEX chromatographic fibers. This results in the efficient retention of large and small particulates without developing a surface cake layer. The media can also remove soluble impurities which results in cleaner effluent than centrifugation or depth filtration.
Key features and benefits:
- Simplification of high cell density cell culture fluid clarification unit operations
- Optimized for high density CHO cell cultures (5 - 8% packed cell volume, PCV)
- Replacement of primary, secondary, and guard membrane clarification stages
- Typical product recoveries of >95% (capsules)
- Synthetic chromatographic harvest media with chemically defined extractables
- Predictable scaling from discovery to manufacturing
