AAV Ratio Kit
The Gator ® AAV Ratio Kit enables empty vs full determination of AAV serotypes 1-10 and chimeric serotypes in crude and purified samples. The AAV Ratio Kit includes: AAVX probes (96 probes/tray) SSB probes (96 probes/tray) Lysis Buffer DNA Detection Solution AAV Ratio Substrate Q Buffer SS DNA Standard Lysis tubes ** Contains temperature sensitive components. Must be shipped overnight. **
Gator® AAV Ratio Kit uses the biolayer interferometry (BLI) technology to determine empty (E) versus full (F) content of AAV. It involves three steps: AAV capture using the AAVX probe, lysis for releasing ss DNA, and capture of ss DNA on to single stranded DNA binding (SSB) probe. The ratio is determined by measuring the amount of ss DNA bound to the SSB probes. The method is compatible with crude samples such as cell lysates and cell culture media. The kit brings automation and speed to empty/full ratio measurement, making it easy to integrate into upstream processes.