AAV1 ELISA Control
1 vial (0.6E+09 – 1.0E+09 capsids)
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The AAV1 ELISA Control consists of fully assembled, empty AAV1 capsids. The concentration is lot specific.
The AAV1 ELISA Control can be used as a positive control with PROGEN's AAV1 Titration ELISA (PRAAV1). If you require different lots of the AAV1 ELISA Control and the Kit Control included in your PROGEN AAV1 Titration ELISA, please enquire to check availability.
Reading of the AAV1 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN's AAV1 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.
The AAV1 ELISA Control has been calibrated on an internally established reference standard. The internal reference standard is a preparation of full AAV1 capsids that has been characterized by qPCR (DNA quantification) and electron microscopy (ratio of full to empty capsids). For further information, please see as an example our poster ‘The New AAV3 Titration ELISA available on aavvirus.com.
Publications
Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17- 24 (2007).