AAVpro® Purification Kit (All Serotypes)

Purification of Adeno-Associated Viral Particles from Virus-Producing Cells: AAVpro Purification Kits

The purity of adeno-associated virus (AAV) particles is important for achieving stable and effective transduction into individual animals and cultured cells. When introducing genes into animals (in vivo transduction), it is necessary to use highly-purified particles that do not contain any residual virus-producing cells or impurities from the culture medium. In addition, when transducing cultured cells, purification of AAV particles eliminates potential negative effects of impurities.

Methods such as cesium chloride concentration gradient ultracentrifugation or iodixanol ultracentrifugation are commonly used to purify AAV particles, but such methods are time-consuming and require careful technique to obtain high yields. The AAVpro Purification Kit (AAV2) uses a column-based purification system to quickly and easily purify AAV serotype 2 particles from virus-producing cells. Use this kit after virus preparation with the AAVpro Helper Free System (AAV2) to generate high yields of highly pure, transduction ready AAV2 particles.

The AAVpro Purification Kit (All Serotypes) uses a precipitation and filtration protocol to purify AAV particles of any serotype.

Features:

• Unique AAV vector extraction methods that eliminate the need for ultracentrifugation
• Kits include all reagents needed to purify AAV particles from vector-producing cells
• Purification yields highly pure AAV particles that can be used directly for in vivo transduction or transduction of cultured cells
• AAVpro Purification Kit (AAV2) is a two-prep kit and does not include Cryonase Cold-Active Nuclease
• AAVpro Purification Kit (All Serotypes) is a four-prep kit and does include Cryonase Cold-Active Nuclease

Applications:

• AAV particle purification from virus-producing cells