Products & ReviewSeparations
ACQUITY UPLC Glycoprotein BEH Amide Column
WatersAvailable: Worldwide
HILIC for Large Biomolecules.
Application Area:
Characterization of Biotherapeutics
This column displays a very high added value for glycoprotein characterisation. Thanks to its large pore size, hydrophilic separation of glycosylated state of whole intact glycoprotein, even antibodies, were rendered possible, robustly and reproducibly.
Review Date: 17 Nov 2016 | Waters
The ACQUITY UPLC Glycoprotein BEH Amide 300Å 1.7 µm Column allows scientists to obtain novel yet complementary glycan related information of biotherapeutic proteins at the intact glycoprotein, glycoprotein subunit, or glycopeptide level.
- Optimized wide-pore, HILIC stationary phase for resolving glycoforms from intact or digested glycoproteins.
- Generation of domain specific glycan linkages with or without MS.
- Elucidation of site specific glycan occupancy.
- High resolution glycopeptide mapping without limitations due to peptide/glycan size or composition.
- Improved resolution in separations of large, released N-glycans (EPO, Factor IX).
- QC tested with Waters Glycoprotein Performance Test Standard.