AdnaTest EMT-2/StemCell
RT-PCR kit for detection of gene expression in enriched tumor cells associated with epithelial-mesenchymal transition and cancer cell stemness.
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AdnaTest EMT-2/StemCell is used for the analysis of EMT (epithelial-mesenchymal transition) and cancer cell stemness characteristics in immunomagnetically-enriched tumor cells. AdnaTest EMT-2/StemCellSelect allows enrichment of circulating tumor cells from peripheral blood, and AdnaTest EMT-2/StemCellDetect allows subsequent analysis of EMT-2/StemCell-associated gene expression. The analysis of breast, colon, prostate and ovarian cancer-associated genes is also possible. The test requires the enrichment of CTCs from 5 ml blood followed by reverse transcription cDNA synthesis using the AdnaTest EMT-2/StemCellDetect and AdnaWash (a specialized wash buffer that reduces leukocyte cross-reactions prior to the multiplex PCR assay).
