AffinityScript Multiple Temperature cDNA Synthesis Kit

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The streamlined protocol produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg of total RNA. The easy-to-use process generates up to 12 kb cDNA in 25 minutes or less. Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format, using SYBR Green or probe-based detection.

Features:

• Optimized for real-time quantitative PCR (qPCR) application
• Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA
• Creates up to 12 kb cDNA in 25 minutes or less
• Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format

For Research Use Only. Not for use in diagnostic procedures.