Products & ReviewLife Sciences
Airway Epithelial Cell Growth Medium 2
Serum- and BPE-free cell culture medium for epithelial cells isolated from large air passages.
Our Airway Epithelial Cell Growth Medium 2 is a serum- and BPE-free formulation optimized for the in vitro cultivation of epithelial cells from large air passages, including HBEpC, HNEpC, and HTEpC.
Key features
- Optimized for in vitro cultivation of epithelial cells from large air passages (HBEpC, HNEpC, HTEpC)
- Serum-free and BPE-free formulation
- Supplied as a 500 ml bottle of Airway Epithelial Cell Basal Medium 2 with premixed SupplementMix
- Contains phenol red
- Average population doubling time (including lag phase) over 3 passages ≤70
Product benefits
- Physiologically relevant growth: Supports cell growth, cobblestone morphology, and functionality that closely mimics the natural in vivo airway environment without the need for small-molecule additives.
- Enhanced proliferation: Promotes higher population doubling across all airway epithelial cell types compared to our standard growth medium.
- Complete BPE-free ALI culture system: Enables isolation, cultivation, and differentiation of bronchial epithelial cells entirely in a serum- and BPE-free environment when used with our Air–Liquid Interface Medium.
- Improved 3D culture integrity: Maintains high and stable TEER values (≥500 Ω·cm²) for at least 14 days.
- Supports primary cell isolation: Proven performance for isolating HBEpC, HNEpC, and HTEpC from fresh bronchial, nasal, and tracheal tissues without additional medium components.
- Functional airway organoids: Supports the formation of rotating spheroid-like structures with predominantly apical-out cilia for ≥28 days when embedded in basal membrane extract.