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Airway Epithelial Cell Growth Medium 2

Serum- and BPE-free cell culture medium for epithelial cells isolated from large air passages.

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PromoCell GmbH

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Our Airway Epithelial Cell Growth Medium 2 is a serum- and BPE-free formulation optimized for the in vitro cultivation of epithelial cells from large air passages, including HBEpC, HNEpC, and HTEpC.

Key features

  • Optimized for in vitro cultivation of epithelial cells from large air passages (HBEpC, HNEpC, HTEpC)
  • Serum-free and BPE-free formulation
  • Supplied as a 500 ml bottle of Airway Epithelial Cell Basal Medium 2 with premixed SupplementMix
  • Contains phenol red
  • Average population doubling time (including lag phase) over 3 passages ≤70

Product benefits

  • Physiologically relevant growth: Supports cell growth, cobblestone morphology, and functionality that closely mimics the natural in vivo airway environment without the need for small-molecule additives.
  • Enhanced proliferation: Promotes higher population doubling across all airway epithelial cell types compared to our standard growth medium.
  • Complete BPE-free ALI culture system: Enables isolation, cultivation, and differentiation of bronchial epithelial cells entirely in a serum- and BPE-free environment when used with our Air–Liquid Interface Medium.
  • Improved 3D culture integrity: Maintains high and stable TEER values (≥500 Ω·cm²) for at least 14 days.
  • Supports primary cell isolation: Proven performance for isolating HBEpC, HNEpC, and HTEpC from fresh bronchial, nasal, and tracheal tissues without additional medium components.
  • Functional airway organoids: Supports the formation of rotating spheroid-like structures with predominantly apical-out cilia for ≥28 days when embedded in basal membrane extract.

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