Amnis® NFkB Translocation Kit
The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus. Nuclear Factor kappa B (NFκB) transcription factor plays a central role in regulating many key processes in mammalian cells, including diff…
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Great product for specialized work with proteins and protein analysis!
p50 heterodimer subunit determination
Good value for the money, very effective among comparable products, and very easy to use. The biggest advantage is the time spent on the whole process which is pretty low.
Review Date: 8 Jun 2021 | Merck KGaA, Darmstadt, Germany
The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus.
Nuclear Factor kappa B (NFκB) transcription factor plays a central role in regulating many key processes in mammalian cells, including differentiation, cell growth, inflammation, immunity, and stress responses. The p50/p65 heterodimer is considered to be the most abundant form of NFκB. NFκB is held in an inactive state in the cytoplasm by its inhibitor IkB. Phosphorylation of serine residues on the IkB proteins marks them for degradation via ubiquitination, therefore allowing activation of the NFκB complex. Activated NFκB complex translocates into the nucleus. The Anti-Hu NFκB Alexa Fluor® 488 antibody recognizes NFκB p50 which is a component of the NFκB p50/p65 heterodimer.The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor® 488, the nuclear stain 7-AAD alonge with the fixation solution, assay buffer and permeabilization buffer. This 2 color imaging flow cytometry kit along with the Amnis imaging cytometry platforms are designed to detect and quantitate NFkB translocation from the cytoplasm to the nucleus.
Applications:
- Flow Cytometry
