Anti-Citrulline (Modified) Detection Kit
This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine…
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Very good product
Provides reagent for the modification and detection of citrulline containing proteins by western blo
The product was quite useful in application and was useful in western blotting
Review Date: 29 Jul 2022 | Merck
This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine in a strong acid solution to form a ureido group adduct. This ensures detection of citrulline-containing proteins regardless of neighboring amino acid sequences. Detection of the modified citrulline proteins is subsequently performed using a standard immunoblot protocol with the anti-Citrulline and goat anti-rabbit IgG-HRP.
