Anti-Poly (ADP-Ribose) Polymerase

Application

Western blot, Immunochemistry, Immunoprecipitation

Product Description

Polyclonal serum
IgG class: rabbit Ig
No. of tests: variable
Species specificity: primates and rodents
Specificity: Poly(ADP-ribose)polymerase (PARP) is a zinc-dependent eukaryotic DNA-binding protein that specifically recognizes DNA-strand breaks that are produced by various genotoxic agents. Recently, it has been reported that PARP serves as a substrate for apoptosis-specific proteases from the ICE family. The protease responsible for the cleavage of PARP was termed apopain, which is derived from its proenzyme Yama/CPP32b.
Additionally, CPP32/Mch2, TX, and Nedd-2 were described as PARP-cleaving ICE-like proteases. It has been found that the 113-kD PARP is cleaved during apoptosis into 89-kD and 24-kD fragments, which could serve as an early specific marker of apoptosis. In immunoprecipitation and western-blot experiments, the antibody recognizes full length or fragmented PARP (primates and rodents).
Working concentration: 0.5 µl/ml (western blot)