ARM-D® Kit, β-Lactamase
The Streck ARM-D Kit, β-Lactamase is a multiplex real-time PCR kit for the rapid detection of β-lactam antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 450 allelic variants in 9 clinically relevant carbapenemases, extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated ampC gene families, KPC, NDM, OXA-48, IMP, VIM, DHA, CMY-2, CTX-M-14 and CTX-M-15.
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Excellent choice to detect b-lactamase-production strains in a Molecular Laboratory.
Molecular Laboratory
We have been using the ARM-D Kit, b-lactamase for a few years in our Lab. The kit is ready to use and contains all the reagents necessary for the test, including the controls. The procedure and results analysis are simple and straight forward. The test is fast, reliable and overall, it represents an excellent choice to detect and identify beta-lactamase-production bacteria strains in a Molecular Laboratory.
Review Date: 23 Jun 2021 | Streck
Good kit for gains of efficiency in the laboratory.
Great multiplexed assay for CRE detection
This kit has simplified our workflow in the laboratory. Previously we were using the CDC designed assay for CRE detection. However, that is a single plex assay that needed more hands-on time. The Streck kit was easy to complete the verification study and is currently in use. For the full fledged panel, this is a good choice.
Review Date: 17 Jul 2020 | Streck
We trust this this test to provide us with reliable and consistent results.
Antibiotic resistance genes testing
Our laboratory has been using the Streck ARM-D PCR kits for beta lactamase gene detection for over two years now. The test is quick, accurate, and easy to use. We can quickly teach our technologists how to run and interpret the test. The test works very well on many PCR platforms, but we prefer the ABI 7500 DX. The PCR itself only takes 35 minutes so starting from a bacterial culture, extracting the DNA, setting up the master mix, and getting a result only takes about 2.5 hours. The nice thing is that the PCR cycling parameters are similar for the other ARM-D tests (ampC, OXA, and mcr). We are therefore able to perform them all on one PCR plate if we need to save time.
Review Date: 24 Jun 2020 | Streck
The ARM-D Kit, β-Lactamase is part of a family of comprehensive, multiplex real-time PCR kits that detect over 1,000 antimicrobial resistance (AMR) gene variants from 26 different gene target families. Streck ARM-D Kits provide a rapid detection strategy for monitoring β-lactam antibiotic resistance. With these kits, your lab can improve surveillance, aid antibiotic stewardship programs and support infection control programs. These kits have a high degree of sensitivity and specificity, providing you accurate, reliable results. Every kit includes positive controls for each gene target to monitor analytical process as well as an endogenous internal control (16SrRNA gene) to reduce false negatives due to PCR inhibition, DNA degradation or poor extraction.
All Streck ARM-D kits are compatible with most 4-channel real-time thermal cyclers. An amplification protocol and analysis guide are available for the following instruments: Applied Biosystems™ 7500 Fast and 7500 Fast Dx, QuantStudio™ 7Flex, Bio-Rad CFX96 Touch™ and QIAGEN Rotor-Gene® Q.
Features
- Detects over 450 allelic variants in the KPC, NDM, OXA-48, IMP, VIM, DHA, CMY-2, CTX-M-14 and CTX-M-15 gene families
- Compatible with most 4-channel real-time PCR thermal cyclers
- Protocol can be completed in less than one hour
- Contains positive controls for each gene target
- Includes a premixed 2X master mix (DNA Polymerase, dNTPs, MgCl2 and reaction buffer)
Benefits
- Detects gene variants not covered by cartridge-based kits
- Identifies resistance mechanisms before susceptibility results are available
- High degree of sensitivity and specificity ensures reliable and accurate results
- Reduced cost per sample compared to cartridge-based methods
- For Research Use Only. Not for use in diagnostic procedures.
