Ascentis® Express U/HPLC Columns
The Fused-Core ® particle technology behind Ascentis ® Express columns delivers maximum speed and efficiency on both HPLC and UHPLC systems. The novel superficially porous silica particles (SPP) are available in three different particle sizes and two different pore sizes for optimal suitability from micro over UHPLC and HPLC to semi-preparative use.
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Great column.
C18
We use the Ascentis® Express C18 (53825-U Supelco) column weekly. It is our go to column for new method development.
Review Date: 9 Feb 2021 | Merck
Ascentis® Express is a high-speed, UHPLC column based on the Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the porous shell. Ascentis® Express columns exceed the benefits of sub2, 3 and 5 µm particles. Ascentis® Express 2.7 µm delivers more resolving power per unit pressure than even sub-2 µm particles on any HPLC system (including UHPLC).
There are three sizes of Ascentis® Express columns:
- 2.0 µm particles: Best Fused-Core® UHPLC column. An optimized solution for high-throughput small molecule analysis.
- 2.7 µm particles: Fast HPLC on any system. A practical solution that delivers UHPLC performance from any HPLC.
- 5.0 µm particles: The lab work-horse column. True plug and play solution for improving existing 3 or 5 μm fully porous particle HPLC columns.
A wide range of column chemistries enable the superior separation of several compound classes, meeting the demands in many different application fields.
Relevant applications:
- UHPLC Analysis of Conjugated Estrogens
- LC-MS/MS Analysis of Epinephrine, Metanephrine and Metabolites
- HPLC Analysis of B-vitamins using UV detection
- HPLC Analysis of 2-AMP and Piroxicam
- HPLC Analysis of Carotene Compounds
