Asp-N, Sequencing Grade
Hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys)
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Great products. But a little bit expensive.
Protein digestion
It gave high-quality results with good reproducibility.
Review Date: 17 Jan 2019 | Promega Corp.
Good product
Protein digestion for MS analysis
Good specificity and works in a broad pH range. Typically a quick digestion works fine.
Review Date: 15 Nov 2017 | Promega Corp.
This endoproteinase hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys). Asp-N activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution or in gel.
Applications:
- Peptide mapping
- Protein identification
