Axygen® AxyPrep MAG Tissue Genomic DNA-Medium
The AxyPrep Mag Tissue gDNA Kit utilizes a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instructions to extract DNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5 mL. Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranes and digest protein. DNA is then immobilized on magnetic particles by…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
The AxyPrep Mag Tissue gDNA Kit utilizes a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instructions to extract DNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5 mL. Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranes and digest protein. DNA is then immobilized on magnetic particles by the addition of a magnetic binding reagent. This differential binding allows the DNA to be easily separated from contaminants using a magnetic field. Contaminants can then be rinsed away using a simple washing procedure.AxyPrep Mag Tissue gDNA Kit can be used in the following applications:
- Restriction enzyme digestion
- DNA sequencing
- Genotyping
- PCR amplification
