BIOne Single-Use Bioreactor System

The BIOne benchtop scale single-use bioreactor (SUB) is for mammalian cell growth, cell therapy, insect cells, and other unique applications performed in a stirred tank bioreactor. The gamma irradiated BIOne SUB is engineered with a disposable headplate welded to a triple-layered liner that can be easily inserted into a non-sterile bioreactor glass vessel, converting it to a disposable SUB within a matter of seconds. Upon media addition, the liner expands and molds to the glass vessel, preserving the geometry of the existing bioreactor.

The BIOne SUB’s liner material emulates the large-scale SUBs used in pilot and commercial biopharma production facilities around the world. Performing R & D studies using the most similar material on the market streamlines your tech transfer and can de-risk your process BEFORE transferring to pilot scale.

CUSTOMIZABLE ABOVE & BELOW THE HEADPLATE

• 2L & 5L Working Volume
• Optional Single-Use pH Probe
• Increased Throughput
• Weldable Tubing
• Flute, Micro & Open Pipe Sparging Options
• Non-Invasive DO & Optional pH Probe Ports
• USP Class VI Materials
• Repligen® ATF Perfusion Tube Compatible
• Animal Derivative-Free
• Low Antioxidant Liner Material
• Compatible with Most Manufacturers’ Glass Vessels and Controllers

DO & pH PROBE PORTS

• Non-Invasive (Amperometric DO/Optical pH)
• PG-13.5 (Optical DO/Electrochemical pH)
• Optional Integrated Single-Use pH Probe

PITCHED BLADE IMPELLER

• Single or Dual Impeller

SPARGING OPTIONS

• Flute Sparger (7 x 1.5 mm hole)
• Micro Sparger (20-40 μm pore size)
• Open Pipe Sparger (1 x 3.0 mm)

LOW ANTIOXIDANT LINER MATERIAL

• Outer Layer (LDPE) – Mechanical Strength
• Middle Barrier Layer (EVOH) – Gas Barrier
• Contact Layer (ULDPE) – Cell Culture Compatible, Biologically Inert