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Biotage Miniprep PhyTip Columns for Plasmid DNA Purification

Lysate Direct PhyTip® Column Technology consists of novel purification columns that are composed of a pipet tip containing plasmid DNA binding resin and a thin, inert frit screen at the end of the tip to retain the resin. The unique design of the column allows for DNA binding directly from cell lysates in the presence of precipitates and debris without sample clearing. By eliminating the need for centrifugation or filtration,…

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Lysate Direct PhyTip® Column Technology consists of novel purification columns that are composed of a pipet tip containing plasmid DNA binding resin and a thin, inert frit screen at the end of the tip to retain the resin. The unique design of the column allows for DNA binding directly from cell lysates in the presence of precipitates and debris without sample clearing. By eliminating the need for centrifugation or filtration, lysate direct PhyTip columns for plasmid DNA purification enable utilization of liquid handing automation found in high throughput laboratories to easily purify up to 96 samples in parallel. In addition, lysate direct PhyTip® columns are also compatible with the MEA Personal Purification System available from PhyNexus for 12-at-a-time sample processing.

Features include:

  • No filtration or centrifugation of lysate required
  • Consistent purification of up to 15 μg
  • Transfection grade and sequencing-ready
  • 96 samples at a time
  • Suitable for transfection, transformation, sequencing, PCR and cloning
  • Fast and fully automated from the cell pellet, no lysate centrifugation required

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