BrightStar™ ECL Detection kit, highly sensitive to Femtogram levels
BrightStar™ Femto Chemiluminescent Substrate Kit
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A useful ECL reagent worth the cost with good quality.
Western Blotting. Visualizing bands using Films or digital imaging system
I have continuously used BrightStar™ ECL Detection kit that is a HRP Chemiluminescent Substrate for my Western Blotting. The product have given me very good data and has helped produce data from blots with very low concentrations of protein. The product is a highly sensitive luminolbased chemiluminescent substrate that employs horseradish peroxidase (HRP). In my experience the reagents seem very sensitive and has given me consistent results. Alkali scientific Inc's price is reasonable and they have an excellent customer service and inter phase. I have tried them directly for antibodies that are weak. They enhance the required band width with minimum background. I have also used them with regular ECL reagents in small proportions. They show up well in both PVDF and nitrocellulose membranes. The value for money is great and this reagent has helped me come up with some good Westerns of publication quality.
Review Date: 15 Nov 2017 | Alkali Scientific
BrightStar™ Femto Chemiluminescent Substrate Kit
• 100pg, 50pg, 25pg, 12.5pg, 6.25pg, 3.13pg • Block 5% Milk/TBST 1 hour • Incubate in conjugated antibody for 2 hours • Goat α-rabbit-HRP 1:10,000 in 5% milk/TBS-T (1/30/14)
• Wash 3x TBST 10 min • Incubate in substrate 5min, drain XS on filter paper
Immobilon lot 1331501 exp 08 JUL 2015
Pierce SuperSignal West Fempo lot OG186675
BrightStar™ 2 component lot 14029A/B 1/29/14
Lot 12249 4°C (From 9/7/12 Stability Assay)
Luminata Forte Lot 133055 exp 01 MAY 2015
BrightStar™ Lot 14029 1/29/14 single component
Expose on cooled CCD camera for 5 minutes at the indicated length of time after addition of the substrate • Analyze images on MutiGauge software for relative signal intensities with background correction
