Buffer XE (45 ml)
For efficient isolation of exosomal RNA from biofluids
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exoRNeasy Kits use membrane affinity spin columns to efficiently capture exosomes and other EVs from most biofluids, including urine, serum, plasma, CSF and cell culture supernatant. The kits then use proven RNeasy MinElute spin columns to isolate RNA from the EVs. The midi column format enables efficient processing of smaller sample volumes (up to 1 ml serum/plasma or 4 ml of urine), while the maxi column format allows the use of large sample volumes, up to 4 ml serum/plasma, 16 ml urine or 32 ml cell culture supernatant, to detect low-abundance RNAs with high confidence. The results are fast, consistent and highly suited to sensitive downstream applications. The RNA isolation part of the protocol using exoRNeasy Kits can be automated on the QIAcube Connect.
