Calmodulin Affinity Resin
The Affinity protein expression and purification system provides a simple, gentle, and effective method for high-level protein expression and purification. A complete Affinity protein expression and purification system includes calmodulin (calcium-modulated protein or CaM) affinity resin and pCAL expression vectors, containing a calmodulin-binding peptide (CBP) tag.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
The Affinity protein expression and purification system provides a simple, gentle, and effective method for high-level protein expression and purification. A complete Affinity protein expression and purification system includes calmodulin (calcium-modulated protein or CaM) affinity resin and pCAL expression vectors, containing a calmodulin-binding peptide (CBP) tag.
The Affinity protein expression vectors pCAL-n, pCAL-n-EK, pCAL-n-FLAG, and pCAL-c, allow fusion of the CBP affinity tag to the N- or C-terminus of the protein-coding sequence of interest. Protein expression is tightly repressed under conditions in which expression is undesirable and high-level induced expression can be achieved. With one pass through calmodulin affinity resin using moderate buffer conditions at neutral pH, calmodulin-binding-peptide fusion proteins can be purified from crude cell extracts to near homogeneity.
The affinity protein expression and purification system allows rapid and efficient purification of calmodulin-binding-peptide (CBP)-tagged fusion proteins from E. coli extracts.
Features:
- For the purification of calmodulin-binding peptide tagged proteins
- Binds CBP-tagged recombinant proteins
- Affinity protein expression vectors pCAL-n, pCAL-n-EK, pCAL-n-FLAG, and pCAL-c are available for purchase separately
- Easy tag removal resulting in native protein.
- Purification tag combines small size, gentle elution and high-affinity binding.
For Research Use Only. Not for use in diagnostic procedures.