Capturem™ His-Tagged Protein Purification Kits

The Next Generation of His-Tagged Protein Purification—Capturem Miniprep Kits

The Capturem His-Tagged Purification Miniprep Kit is a revolutionary solution for recombinant protein purification. This complete system effortlessly outperforms conventional methods:

• Spin columns with high-capacity membranes instead of resin
• Elute concentrated protein: up to 0.3–1 mg/ml
• Speed: 5 min at room temperature instead of 1–2 hr in the cold room
• Flexibility: purify from mammalian or bacterial cells

Where resin columns fall short, this new system provides high capacity and purity in just five minutes, from loading sample application through elution. Novel nylon membranes have been assembled into spin columns, creating a no-waiting, room-temperature workflow compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of research settings, including denaturing conditions, in the presence of additives (e.g., βME, EDTA, DTT, glycerol, TCEP, etc.; see table below), and when performing tandem purification of his-tagged proteins.
The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein, due to the chemically enhanced surface area of the pores. With this technology, protein binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane. A five-minute protocol performed entirely at room temperature makes for a convenient, powerful, and efficient research tool.

Each disposable miniprep spin column has a sample capacity of up to 800 µl, and produces ~100 µg/column at a concentration of ~0.3 mg/ml. The short residence time of protein samples greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.

Features:

• No-waiting workflow—5-minute, room-temperature protocol
• High purity and yield—Approximately 100 µg/column, at a concentration of ~0.3–1 mg/ml
• Wide range of conditions—Native and denaturing conditions; with common additives (e.g., EDTA, DTT, βME, glycerol, TCEP, etc.)
• Lysis buffer compatibility—xTractor Buffer, buffers from other vendors
• Different cell systems—Easy purification from mammalian and bacterial cells

Applications:

• Biochemical/enzymatic assays
• Pull-down or other protein interaction assays
• Confirmation screening
• Protein/ligand affinity analysis
• Crystallography or other structural analyses
• Antibody production
• Toxicology studies
• Protein transfer to live cells