Capturem™ IP & Co-IP Kit

Rapid Protein A-based IP—Capturem IP & Co-IP Kit

Immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) are valuable tools for studying the interaction of proteins with macromolecules, such as other proteins. The Capturem IP & Co-IP Kit provides a complete, easy-to-use solution for the rapid isolation of individual proteins or protein-protein complexes. The kit features Capturem Protein A columns for high-capacity, membrane-based purification of antibody-bound protein complexes. All of the buffers necessary for cell lysis as well as binding, washing, and eluting of the antibody-antigen complexes are also included in the kit. IPs are performed in just 15 minutes, from sample incubation with antibody through elution.

Features:

• Simple, ultra-fast workflow: incubate IP antibody with sample for 10 minutes then purify at room temperature in 5 minutes
• Complete solution: includes Capturem Protein A spin columns and immunoprecipitation buffers optimized for IP and Co-IP experiments
• High concentration: extremely small elution volumes yield highly concentrated complexes
• High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
• Versatile: compatible with a wide range of IP buffers and conditions
• Single-use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

Applications:

• Immunoprecipitation
• Co-immunoprecipitation
• Investigation of protein complexes
• Antigen screening for antibody discovery and engineering workflows