Capturem™ Protein G Antibody Purification Kits

Rapid Antibody Purification Kits—Capturem Protein G Miniprep Kits

Capturem Protein G Miniprep columns combine the specific antibody binding properties of Protein G with Capturem technology to provide rapid, small-scale, high-quality purification and screening of monoclonal and polyclonal antibodies. These columns are convenient, powerful, and efficient antibody purification tools that offer the following advantages:

• Fast and convenient: purify antibodies in 5 minutes at room temperature
• High capacity: columns and plate wells bind ≥75 mg/ml of antibody
• High purity: due to short residence times and an enhanced purification protocol
• Flexible: purify a wide range of antibodies

Protein G is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the Fc region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, human, etc. This product uses a recombinant Protein G that was engineered to remove the albumin binding domain, as well as the cell wall and cell membrane binding domains, in order to improve specificity towards the Fc region of antibodies. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, 96-well plates, and large-volume units.

Features:

• No-waiting workflow: 5-minute, room-temperature protocol
• High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
• High concentration: extremely small elution volumes yield highly concentrated antibody
• High quality: short residence times reduce the possibility of degradation or loss of activity
• Versatile: purify a wide range of antibodies
• Single use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover

Applications:

• Antibody purification
• Antibody screening
• Immunoprecipitation