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Cas9 sgRNA vector
Cas9 sgRNA vector for cloning
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Application Area:
Reprogramming cells
It is a great CRISPR based tool, and I'm happy with Addgene's service - fast, kind and very professional. The waived fee is also available.
Review Date: 8 Feb 2021 | Addgene
- Vector backbone: gRNA cloning vector (Addgene plasmid #41824)
- Backbone size (bp): 3900
- Modifications to backbone: A previously described chimeric guide RNA expression cassette was ordered as gBlocks and cloned into the gRNA cloning vector (Addgene plasmid #41824). The new sgRNA cloning vector (Cas9 sgRNA vector) includes two BbsI restriction sites for rapid cloning of sgRNA
- Vector type: Mammalian Expression, CRISPR