Cell Proliferation ELISA, BrdU (colorimetric)

Application

The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:

• Detection and quantification of cell proliferation induced by growth factors and cytokines
• Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
• Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
• Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
  
  Benefits
  
• Safe: No radioactive isotopes are used.
• Accurate: Results obtained strongly correlate to the number of proliferating cells (low mean deviation).
• Sensitive: Colorimetric technology provides sensitivity that is at least equivalent to the [3H]-thymidine incorporation assay.
• Fast: Fixation and denaturation in a single step, one washing and two incubation steps only; the use of a multiwell ELISA reader allows a larger number of samples to be processed.
• Convenient: Reagents are provided in a stable and optimized form; the entire assay is performed in one microplate; mild fixation and DNA denaturation preserve cellular morphology.
• Function-tested: Every lot is function-tested on proliferating cells in comparison to a master lot.