Chemical Tagging Reagents

Chemical tagging involves chemically modifying an analyte, such as a glycan or a peptide, through a defined reaction prior to analytical analysis. The reaction may take the form of reductive methylation of primary amines in peptides (via formaldehyde and cyanoborohydride in their unlabeled and stable isotope-labeled forms) or hydrazide tagging of enzymatically released N-glycans (via phenyl 2-GPN). The latter glycan tagging kit is termed Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT^®). All reductive methylation reactions described are designed for 2- or 3-plex relative quantitation studies.