Chromatrap® Native ChIP Kit
Chromatrap® Native ChIP kits provide an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction.
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Chromatrap® Native ChIP kits provide an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction.
In native chromatin the proteins and histones wrapped around DNA are naturally linked and the traditional methods of shearing can easily disrupt the DNA- protein complexes. Therefore the DNA is biologically sheared using enzyme digestion of the chromatin. This kit can be can be used for the study of histone modifications and certain abundant transcription factors that are likely to be bound to DNA.
Chromatrap® Native ChIP kits provide a quicker, easier and higly sensitive way of performing ChIP assays from native chromatin.
Features and Advantages of Chromatrap ChIP-Seq Kit:
- ChIP in under 5 hours
- ChIP from as little as 1000 cells or up to 50ug DNA
- Perfectly orientated proteins for maximum complex binding
- Inert system for background elimination
The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
