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CloneAmp™ HiFi PCR Premix

High Fidelity PCR for Cloning—CloneAmp HiFi PCR Premix

Takara Bio

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High Fidelity PCR for Cloning—CloneAmp HiFi PCR Premix

CloneAmp HiFi PCR Premix is a convenient 2X master mix that provides exceptionally accurate and efficient DNA amplification, making it ideal for In-Fusion PCR cloning. It contains dNTPs and optimized buffer, allowing rapid set-up of PCR reactions and facilitating high-throughput applications.

Features:

  • Highly accurate—exceptionally low error rate (12 mismatched bases per 542,580 total bases)
  • Fast—quick priming and extension (5 sec/kb) enable fast PCR reactions
  • Specific—hot start antibody prevents nonspecific amplification
  • Versatile—works with long (up to 10 kb) and GC-rich templates
  • Convenient—premix allows rapid reaction set-up

Applications:

  • In-Fusion PCR cloning

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