Corning® Matrigel® Basement Membrane Matrix, LDEV-free, 50 mL, 1/Pack, 5/Case
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.Quality Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extens…
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Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
- Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
- Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
- Tested and found negative for bacteria, fungi, and mycoplasma
- Protein concentrations are determined by Lowry method
- Endotoxin units are measured by Limulus Amoebocyte Lysate assay
- BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
- Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor.
Source
Engelbreth-Holm-Swarm Mouse Tumor
Application
- Cell Growth and Differentiation
- Metabolism/Toxicology Studies
- Invasion Assays
- in vitro and in vivo Angiogenesis Assays
- in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Features
- Certified LDEV-free
- Extensive quality control exceeding industry standard
- Rigorously validated for functionality
- Most extensively references basement membrane matrix
Click here for Corning Matrigel Lot Selection and Reservation.
For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.
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