Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free, 10 mL
Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 10 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins…
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Great results
Cell invasion assay
It is a good product
Review Date: 8 Jul 2022 | Corning Life Sciences
Best extracellular matrix to culture intestinal organoids.
3D organoid culture
Enteroids growth in a consistent manner and keep the same morphology over the passages. When compared with other ECM from animal origin, enteroid growth in matrigel is more robust and reproducible. Use of matrigel LOTs with low level of endotoxins is preferable. So sometimes you need to wait some time to get a good LOT. Use of matrigel requires some attention since it needs to be manipulated always on ice to avoid early polymerization.
Review Date: 19 Nov 2021 | Corning Life Sciences
Perfect matrigel for intestinal organoids!
Organoids
We have been using this matrigel for intestinal organoids culture and they have been working really well. The viscosity is suitable to form a dome in the wells and make a perfect matrigel pad for intestinal organoids. It is totally transparent and solidifies pretty easily when put in the incubator for 5 minutes! I definitely recommend this for organoid culture!
Review Date: 23 Mar 2021 | Corning Life Sciences
It is a very good product!
Generate patient-derived organoids
I use Matrigel all the time to generate patient-derived organoids. The matrigel I purchased from Corning has stable quality and it makes my experiments easy to handle and repeatable!
Review Date: 23 Mar 2021 | Corning Life Sciences
Essential product for production of cerebral organoids!
Coat surfaces for making cerebral organoids
Very easy to use. Creates perfect matrix for growing primary cells and for use as a scaffold for cerebral organoids. It is important to limit freeze/thaw cycles. Aliquoting is integral to a successful process.
Review Date: 5 Feb 2019 | Corning Life Sciences
Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 10 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
- Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
- Extensive PCR testing performed to screen for a number of pathogens
- Tested and found negative for bacteria, fungi, and mycoplasma
- Protein concentrations determined by Lowry method
- Endotoxin units measured by Limulus Amoebocyte Lysate assay
- Gel stability tested for a period of 14 days at 37°C
- Biological activity determined for each lot using neurite outgrowth assay
Source
EHS mouse tumor
Application
- Cell Growth and Differentiation
- Metabolism/Toxicology Studies
- Invasion Assays
- in vitro and in vivo Angiogenesis Assays
- in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws.
For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.
