Crypto/Giardia/Entomoeba Ag strip test

Crypto/Giardia/Entamoeba Ag Rapid Test (strip) and Crypto/Giardia/Entamoeba Ag Rapid Test (cassettes) chromatographic immunoassay is a procedure for in vitroqualitative detection of Cryptosporidium parvum, Giardia lamblia and Entamoeba histolytica antigens in human stool samples.

The tests are based on the use of three types of microspheres: blue microspheres covalently linked to a monoclonal anti-Cryptosporidium parvum antibody, red microspheres covalently linked to a monoclonal anti-Giardia lamblia antibody, green microspheres covalently linked to a monoclonal anti-Entamoeba histolytica antibody as well as purple microspheres used as test control. The parasites present in stool samples react with the latex particles which are coated with specific monoclonal antibodies against the antigen. This latex particles/antibodies/parasite complex migrates through a chromatographic process towards the reaction area. In this area, anti-Cryptosporidium, anti-Giardia and anti-Entamoeba antibodies that react with the latex particles/antibodies/parasite complex are present. This reaction leads to the appearance of a blue and/or red and/or green lines. These lines are used to interpret the result, following a ten-minute room-temperature incubation.