Crystal Structure Determination Service
1. Service for crystallization and structure determination We provide gene-to-structure services in our laboratory. Our hands-on Ph.D. scientists draw on years of experience that allow them to select proper ways to express and purify a clients’ target proteins. It is our standard to prepare the best quality protein sample in purity, activity and stability for crystallization study. All crystal structures at SBD will be d…
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Highly recommended, great service!
Facilitate XRD analysis and structure determination
Structure Based Design was instrumental in getting our crystals to the Stamford beamline and helping us solve crystal structures.
Review Date: 25 Oct 2018 | Structure Based Design, Inc.
1. Service for crystallization and structure determination
We provide gene-to-structure services in our laboratory. Our hands-on Ph.D. scientists draw on years of experience that allow them to select proper ways to express and purify a clients’ target proteins. It is our standard to prepare the best quality protein sample in purity, activity and stability for crystallization study.
All crystal structures at SBD will be determined with the data sets collected using synchrotron light sources. The quality of the final X-ray structure will match the publication requests for a crystal structure. Almost all protein crystals obtained at SBD have a higher resolution than the published crystal structure of same protein.
Our crystallographers have experience solving various drug targets including Kinases, Nuclear Receptors, Phosphodiesterases, Polymerases, Dehydrogenases, Proteases, RNA, Protein/protein complex and Protein/RNA complex et al.
2. Service for co-crystal structure determination with your leads
SBD has proprietary techniques in obtaining co-crystal structures in complex with leads via co-crystallization or soaking method. If the IC50 value of your lead is better than 50 μM, we will get the co-structure for you. Finding a compound in the co-structure not only depends on its affinity, but also on its solubility, specific interaction, and location of the binding site. More important is the art of co-crystallization and soaking skills. As discussed in the technology section, our experience in this field will enable the best chance at finding your compound in the co-crystal structure. We offer co-crystal structure services for hard-to-reach projects including tough protein targets and weak binders.
Location and refinement of our client’s compounds is the crucial step of a co-crystal structure. SBD will provide the highest quality co-crystal structure. The electron density map of the ligand in a complex structure will be well refined in order to precisely determine the position of all atoms.
3. Service models and packages
SBD’s service model is customer driven. We have flexible service packages to meet a client’s individual needs.
Service package I:
1.Crystallization of target protein provided by client 2. Determination of apo-crystal structure 3. Determination of co-crystal structures with ligands
Service package II:
1. Protein expression and purification at SBD 2. Multiple expression constructs of protein may be designed to obtain high resolution crystal 3. Determination of apo-crystal structure with a resolution of at least 2.5 Å 4. Determination of co-crystal structures with ligands
Service package III
1. Develop a high throughput protocol from protein preparation to co-crystal determination in order to efficiently obtain a number of co-crystal structures 2. Determine 20-30 co-crystal structures to support full processing of a client’s drug discovery project
