Crystal Violet
Certified by the Biological Stain Commission
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The best cell viability method!
Viability of cells
Crystal violet was used to evaluate cells viability instead of the classic MTT method. The cells are fixed in 4% paraformaldehyde, washed with PBS, incubated in 0.1% of Crystal violet for 60 minutes at RT. After washing with water, the color is dissolved in 33% of acetic acid. The method allows a good cells viability evaluation without the possible interference of metabolic processes that affect MTT.
Review Date: 18 Dec 2018 | Merck
Packaging
25, 100 g in glass bottle
Application
Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion
Biochem/physiol Actions
Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes.Crystal Violet also helps in colorimetric measurement of cell viability.
Suitability
Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.
