Desmin, chicken gizzard, 100 µg
Protein standard for immunoblotting, immunization and immunoassays.
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Protein standard for immunoblotting, immunization and immunoassays.
Reconstitution to filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M and Franke WW (1985). J Cell Biol 101, 1826-1841
- Hatzfeld M et al. (1987). J Mol Biol 197, 237-255
