E.Z.N.A.® Endo-Free Plasmid DNA Maxi Kit
Isolate low endotoxin (<1EU/µg) plasmid Dna from up to 200 mL bacterial cultures using spin maxi columns
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Endotoxins are lipopolysaccharides (LPS), found in the outer cell membrane of gram negative bacteria such as E. coli. One E. coli cell contains around 2 million LPS molecules, each having hydrophobic, hydrophilic, and charged regions. Bacteria release small quantities of endotoxins during growth, and large quantities at death. At the time of lysis during plasmid purification, endotoxins are shed into the lysate. The chemical and physical properties that endotoxin molecules possess lead to their co-purification with plasmid DNA by behaving similarly on the surface of silica and anion-exchange resins. The E.Z.N.A.® Endo-Free Plasmid System uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiBind® matrix. In addition, the E.Z.N.A.® Endo-Free Plasmid Maxi Kit includes specialized filter cartridges that replace the centrifugation step following alkaline lysis.
