E.Z.N.A.® FastFilter Plasmid DNA Mini Kit
Isolate Plasmid DNA from up to 5 mL culture in 9 minutes utilizing an innovative lysate clearance column
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The E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA from up to 5 mL of culture following alkaline-lysis method. The kit features an innovative and first of its kind FastFilter Mini Column that is nested within a regular HiBind® DNA Mini Column to combine lysate clearance and DNA binding into one or two centrifugation steps depending on the culture volume. The protocol time is just 9 minutes when starting with bacterial pellet from up to 3 mL culture volume. The protocol time for culture volumes greater than 3 mL will be slightly longer due to two centrifugation steps for lysate clearance. Following wash and elute steps, purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing.
