E.Z.N.A.® FastFilter Plasmid DNA Mini Kit

The E.Z.N.A.^® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA from up to 5 mL of culture following alkaline-lysis method. The kit features an innovative and first of its kind FastFilter Mini Column that is nested within a regular HiBind^® DNA Mini Column to combine lysate clearance and DNA binding into one or two centrifugation steps depending on the culture volume. The protocol time is just 9 minutes when starting with bacterial pellet from up to 3 mL culture volume. The protocol time for culture volumes greater than 3 mL will be slightly longer due to two centrifugation steps for lysate clearance. Following wash and elute steps, purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing.