E.Z.N.A.® MicroElute Total RNA Kit
Isolates RNA from small samples with lower elution volumes using spin columns
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Quick, easy, and great 260/280 values!
RNA isolation from human primary cells
I had been struggling with RNA extraction for a while from such a low number of cells I use in my experiments; however, the RNA that I isolate from this kit usually have super high 260/280 ratios, which means I'm getting very high quality RNA. I have been able to use the RNA I isolate from this kit for cDNA synthesis and qPCR and it's worked well, even if I do have low concentrations for some samples.
Review Date: 8 Nov 2019 | Omega Bio-Tek
E.Z.N.A. ® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as laser dissected samples (LDS) or fine-needle aspirates (FNA). Normally, up to 5 x 105 eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows the processing of single or multiple samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10-15 µL nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, poly A+ purification, nuclease protection and in vitro translation.
