ECFG21 Super Electro-Cell Fusion Generator
Dramatically Higher Efficiency than PEG
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The spontaneous fusion of somatic cells in a test tube occurs less than 1 in 1,000,000 times. Though this is good if you are a living organism, it can be inconvient for specialized applications. Historically, a chemical such as PEG has been used to increase spontaneous fusion by two or three orders of magnitude, or inactivated viruses have been used to promote the fusion of proximal cells. More recently, protocols utilizing AC and DC electric fields have further improved the efficiency of cell fusion – in some cases, by as much as 25- or 100-fold.
The ECFG21 incorporates the latest in solid state electronic design for a robust system with plenty of useful feedback. The three-phase technique relies upon an AC field to physically align the cells end-to-end in a chain formation. Once in physical contact, the system applies a series of DC pulses to disrupt the plasma membrane and induce fusion of adjacent cells. The final AC phase ensures that as many as one in every hundred cells makes a viable hybrid cell with two nuclei. The nuclei later fuse and the process completes. Only the ECFG21 can provide real-time feedback of voltage and current to ensure that every sample is under optimal conditions for cell fusion. The ECFG21 precisely controls AC to DC switching time, DC pulsing amplitude and length (including a convenient reverse polarity option), post-fusion AC current for maintenance of the end-to-end cell configuration, and manual control of DC pulsing to enable observation of first-time cell optimizations under a microscope.
You don’t have to take our word for it, either—reviews of this new system have been tremendous. The ECFG21 is consistently reported to provide much better control of experimental conditions as compared with chemical and viral methods. Electro-cell fusion typically produces hybrid cell lines that are more stable, too, in part due to the lower cytotoxicity inherent to the precise electric field and pulsing control of this instrument. Results tend to be more reproducible and repeatable for the same reason. We have an experienced team of specialists here at Bulldog, and we will work closely with you to optimize your results. Give us a call and start fusing immediately!
