Endoglycosidases
Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates.
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Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked.
Features:
- These products are most commonly used to remove glycans for analysis, or to obtain deglycosylated proteins for analysis.
- Notable among these these is PNGase F, an asparagine amidase that releases intact N-glycans by cleaving between the innermost GlcNAc of the N-glycan and the asparagine (Asn) residue of the peptide the N-glycan is linked to.
