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EpiMark® Hot Start Taq DNA Polymerase

New England Biolabs Inc.M0490SAvailable: Worldwide

Taq DNA Polymerase optimized for epigenetics analysis

New England Biolabs Inc.

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Average Rating 5.0

|1Scientists have reviewed this product

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Great results in PCR with CG-rich DNA.

 

Average Rating 5.0

Application Area:

Polymerase Chain Reaction (PCR)

Very effective in our PCR for bisulfite converted DNA and also CG-rich DNA.

Review Date: 24 Jan 2020 | New England Biolabs Inc.

EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA.

Taq DNA polymerase possesses a 5´- 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.

Taq DNA Polymerase optimized for epigenetics analysis

  • Ideal for use on bisulfite-converted DNA and AT-rich templates
  • Specially formulated reaction buffer system
  • Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup

Applications:

  • Bisulfite Sequencing
  • Hot Start PCR
  • Specialty PCR
  • Routine PCR
  • PCR

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