Products & ReviewLife Sciences
EZ-Link™ NHS-LC-LC-Biotin
Thermo Fisher ScientificAvailable: Worldwide
Thermo Scientific EZ-Link NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for intracellular labeling.
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Reliable reagent
Application Area:
Protein interactions
Very good reagent for general random biotinylation to analyze protein interactions. Can be stored in small aliquots and used with good results for many years.
Review Date: 1 Oct 2021 | Thermo Fisher Scientific
Features of EZ-Link NHS-LC-LC-Biotin:
- Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
- Membrane-permeable—can be used to label inside cells (intracellular)
- Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
- Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
- Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
- Long reach—spacer arm (total length added to target) is 30.5 angstroms; it consists of the biotin valeric acid group extended by a 13-atom chain
